Anticorps Monoclonal anti-TCF3

• Phare
• Validé par KD/KO

TCF3 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D12E5

N° de cat : 67140-1-Ig

Synonymes

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

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Galerie de données de validation

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  WB analysis using 67140-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 67140-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67140-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67140-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 67140-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67140-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 67140-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67140-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67140-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67140-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 67140-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TCF3 antibody (67140-1-Ig, Clone: 1D12E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 67140-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67140-1-Ig (TCF3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules Jurkat, cellules K-562, cellules NCI-H1299, cellules PC-3, cellules THP-1
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67140-1-Ig cible TCF3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TCF3 Protéine recombinante Ag15734
• Nom complet: transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Masse moléculaire calculée: 654 aa, 68 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC110580
• Symbole du gène: TCF3
• Identification du gène (NCBI): 6929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Reproduction; TCF3 regulates human endometrial stromal cell proliferation and migration in RPL.; Authors - Xiao-Wei Wei ; KD Validated
human	WB,IHC	Mol Med Rep; Expression of TCF3 in Wilms' tumor and its regulatory role in kidney tumor cell viability, migration and apoptosis in vitro.; Authors - Nian Zhou ; KD Validated
human	WB	Cancer Med; Palladin promotes cancer stem cell-like properties in lung cancer by activating Wnt/Β-Catenin signaling; Authors - Xiong Shu