Anticorps Monoclonal anti-TCF3

• Phare
• Validé par KD/KO

TCF3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D12E5

N° de cat : 67140-1-PBS

Synonymes

bHLHb21, E2A, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

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Galerie de données de validation

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  WB analysis using 67140-1-Ig (same clone as 67140-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67140-1-Ig (same clone as 67140-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67140-1-Ig (same clone as 67140-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67140-1-Ig (same clone as 67140-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67140-1-Ig (same clone as 67140-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 67140-1-Ig (same clone as 67140-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67140-1-Ig (same clone as 67140-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67140-1-Ig (same clone as 67140-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67140-1-Ig (same clone as 67140-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67140-1-Ig (TCF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TCF3 antibody (67140-1-Ig, Clone: 1D12E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67140-1-Ig (same clone as 67140-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67140-1-Ig (TCF3 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67140-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67140-1-PBS cible TCF3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TCF3 Protéine recombinante Ag15734
• Nom complet: transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Masse moléculaire calculée: 654 aa, 68 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC110580
• Symbole du gène: TCF3
• Identification du gène (NCBI): 6929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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