Anticorps Polyclonal de lapin anti-TCF1/TCF7

• Phare
• Validé par KD/KO

TCF1/TCF7 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(24)

N° de cat : 14464-1-AP

Synonymes

TCF7, TCF-1, TCF1, TCF 7, TCF 1

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Galerie de données de validation

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  WB analysis of mouse thymus using 14464-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14464-1-AP (TCF7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14464-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14464-1-AP (TCF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse spleen using 14464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TCF1/TCF7 antibody (14464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 20 publications below
• IHC: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

14464-1-AP cible TCF1/TCF7 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF1/TCF7 Protéine recombinante Ag5792
• Nom complet: transcription factor 7 (T-cell specific, HMG-box)
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC048769
• Symbole du gène: TCF1/TCF7
• Identification du gène (NCBI): 6932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol; TRIM27 maintains gut homeostasis by promoting intestinal stem cell self-renewal; Authors - Jing Wang
human	WB,IHC	Clin Cancer Res; Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.; Authors - Huanjun Wang ; KD Validated
human,mouse	WB	Int J Biol Macromol; Correlation between TCF7 and bladder cancer and feasibility of Erlotinib targeting in bladder cancer: Molecular mechanism and expression of TCF7 recombinant protein; Authors - Zengmi Mo
human	WB	Clin Transl Med; Long noncoding RNA LINC01606 protects colon cancer cells from ferroptotic cell death and promotes stemness by SCD1-Wnt/β-catenin-TFE3 feedback loop signalling.; Authors - Yajun Luo
mouse	WB	iScience; Suppression of preadipocyte determination by SOX4 limits white adipocyte hyperplasia in obesity; Authors - Ting He
human	WB	J Transl Med; Forkhead Box S1 mediates epithelial-mesenchymal transition through the Wnt/β-catenin signaling pathway to regulate colorectal cancer progression.; Authors - Liang Zhang

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