Anticorps Polyclonal de lapin anti-TCF7L1

TCF7L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14519-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 14519-1-AP (same clone as 14519-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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  WB analysis using 14519-1-AP (same clone as 14519-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14519-1-AP (same clone as 14519-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14519-1-AP (TCF7L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14519-1-AP (same clone as 14519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14519-1-AP (same clone as 14519-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14519-1-AP (TCF7L1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 14519-1-AP (same clone as 14519-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TCF7L1 antibody (14519-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14519-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14519-1-PBS cible TCF7L1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCF7L1 Protéine recombinante Ag5983
• Nom complet: transcription factor 7-like 1 (T-cell specific, HMG-box)
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 63-70 kDa
• Numéro d’acquisition GenBank: BC058894
• Symbole du gène: TCF7L1
• Identification du gène (NCBI): 83439
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TCF7L1 is one of TCF/LEF transcription factors that are mediators of the Wnt signaling pathway and are antagonized by the TGF-beta signaling pathway. It binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. It's necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis

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