Anticorps Polyclonal de lapin anti-TCIRG1

• Phare
• Validé par KD/KO

TCIRG1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12649-1-PBS

Synonymes

T-cell immune regulator 1, OPTB1, OC-116 kDa, OC116, ATP6V0A3

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Galerie de données de validation

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  WB analysis using 12649-1-AP (same clone as 12649-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 12649-1-AP (same clone as 12649-1-PBS)

  WB result of TCIRG1 antibody (12649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCIRG1 transfected A431 cells. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 12649-1-AP (same clone as 12649-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12649-1-AP (same clone as 12649-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12649-1-AP (TCIRG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12649-1-AP (same clone as 12649-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using TCIRG1 antibody 12649-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12649-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12649-1-PBS cible TCIRG1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCIRG1 Protéine recombinante Ag3378
• Nom complet: T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
• Masse moléculaire calculée: 830 aa, 93 kDa
• Poids moléculaire observé: 92 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: BC018133
• Symbole du gène: TCIRG1
• Identification du gène (NCBI): 10312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TCIRG1, also known as V ATPase 116 kDa isoform a3, TIRC7, OPTB1, belongs to the V-ATPase 116 kDa subunit family, TCIRG1 seems to be directly involved in T-cell activation (PubMed:10329006). Alternative splicing results in multiple transcript variants. Isoform long is highly expressed in osteoclastomas. Isoform short is highly expressed in thymus (PMID: 15809087, 24753205). Mutations in TCIRG1 are associated with Osteopetrosis, autosomal recessive 1 (OPTB1)( PMID: 11532986).

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