Anticorps Polyclonal de lapin anti-TCN2

TCN2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 12157-1-AP

Synonymes

D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

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Galerie de données de validation

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  WB analysis using 12157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 12157-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 12157-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12157-1-AP (TCN2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12157-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12157-1-AP (TCN2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules MCF-7, cellules MG U-87, tissu d'intestin grêle de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu d'intestin grêle de souris, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:600
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12157-1-AP cible TCN2 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCN2 Protéine recombinante Ag2825
• Nom complet: transcobalamin II; macrocytic anemia
• Masse moléculaire calculée: 400 aa, 44 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC011239
• Symbole du gène: TCN2
• Identification du gène (NCBI): 6948
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

Publications

Species	Application	Title
canine, cat	IHC	Oncotarget; Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues.; Authors - Annette M Sysel
mouse	IF	Int J Biol Macromol; CD320 expression and apical membrane targeting in renal and intestinal epithelial cells.; Authors - Yue Chen
human	IHC	Anticancer Res; Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts.; Authors - Sysel Annette M AM
rat	WB	Mol Pharm; Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin; Authors - Yuri Kinoshita

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joseph (Verified Customer) (09-17-2020)

We evaluated several antibodies against TCN2 and found the Proteintech sequence to provide the best sensitivity and specificity compared to the other companies

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50 dilution human; 1:50 dilution canine; 1:25 dilution feline
• Cell Tissue Type: human, canine and feline malignant various

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