Anticorps Polyclonal de lapin anti-TCOF1

• Phare
• Validé par KD/KO

TCOF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 11003-1-AP

Synonymes

treacle, MFD1

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Galerie de données de validation

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  WB analysis of Jurkat using 11003-1-AP

  WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.

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  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 11003-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11003-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11003-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11003-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 11003-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (11003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules Neuro-2a
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Informations sur le produit

11003-1-AP cible TCOF1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCOF1 Protéine recombinante Ag1447
• Nom complet: Treacher Collins-Franceschetti syndrome 1
• Masse moléculaire calculée: 97 kDa, 144 kDa, 152 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC014559
• Symbole du gène: TCOF1
• Identification du gène (NCBI): 6949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

Publications

Species	Application	Title
human	WB	Nature; Cell-fate determination by ubiquitin-dependent regulation of translation.; Authors - Achim Werner
human		Nat Commun; Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.; Authors - Stephen M Rawlinson
human	WB, IF	Proc Natl Acad Sci U S A; Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.; Authors - Alberto Ciccia ; KD Validated
human	WB	Elife; Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.; Authors - Achim Werner
human	WB, IF	Int J Mol Sci; TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.; Authors - Ahmed Hassan
human	WB,IF	Cancer Sci; TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.; Authors - Chaoshen Wu

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