Anticorps Recombinant de lapin anti-TCOF1

TCOF1 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242201E12

N° de cat : 84914-4-PBS

Synonymes

242201E12, MFD1, treacle

---

Galerie de données de validation

• 

  WB analysis using 84914-4-RR (same clone as 84914-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84914-4-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 84914-4-RR (same clone as 84914-4-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 84914-4-RR (TCOF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84914-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84914-4-RR (same clone as 84914-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84914-4-RR (TCOF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84914-4-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 84914-4-RR (same clone as 84914-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-4-RR, Clone: 242201E12 ) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84914-4-PBS in a different storage buffer formulation.

---

Informations sur le produit

84914-4-PBS cible TCOF1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TCOF1 Protéine recombinante Ag1447
• Nom complet: Treacher Collins-Franceschetti syndrome 1
• Masse moléculaire calculée: 97 kDa, 144 kDa, 152 kDa
• Poids moléculaire observé: 220-240 kDa
• Numéro d’acquisition GenBank: BC014559
• Symbole du gène: TCOF1
• Identification du gène (NCBI): 6949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

{{ptg:RelatedPrimaryAntibodies}}