Anticorps Recombinant de lapin anti-TCOF1

TCOF1 Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242201F12

N° de cat : 84914-5-PBS

Synonymes

242201F12, MFD1, treacle

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Galerie de données de validation

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  IHC staining of human colon cancer using 84914-5-RR (same clone as 84914-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84914-5-RR (TCOF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84914-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84914-5-RR (same clone as 84914-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-5-RR, Clone: 242201F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84914-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84914-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84914-5-RR against Human TCOF1 were performed. The affinity constant is 8.67 nM.

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Informations sur le produit

84914-5-PBS cible TCOF1 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TCOF1 Protéine recombinante Ag1447
• Nom complet: Treacher Collins-Franceschetti syndrome 1
• Masse moléculaire calculée: 97 kDa, 144 kDa, 152 kDa
• Numéro d’acquisition GenBank: BC014559
• Symbole du gène: TCOF1
• Identification du gène (NCBI): 6949
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

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