Anticorps Polyclonal de lapin anti-TCP1

TCP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10320-1-AP

Synonymes

CCT alpha, CCT1, CCTa, D6S230E, t complex 1, TCP 1 alpha, TCP1

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Galerie de données de validation

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  WB analysis using 10320-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10320-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10320-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10320-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10320-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10320-1-AP (TCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10320-1-AP

  IP Result of anti-TCP1 (IP:10320-1-AP, 4ug; Detection:10320-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10320-1-AP (TCP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10320-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10320-1-AP (TCP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10320-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TCP1 antibody (10320-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10320-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10320-1-AP (TCP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10320-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TCP1 antibody (10320-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, tissu cardiaque de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du côlon humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10320-1-AP cible TCP1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TCP1 Protéine recombinante Ag0389
• Nom complet: t-complex 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000665
• Symbole du gène: TCP1
• Identification du gène (NCBI): 6950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
human	WB	J Cell Biol; CEP55 promotes cilia disassembly through stabilizing Aurora A kinase.; Authors - Yu-Cheng Zhang
mouse,human	WB	Front Cell Dev Biol; TRiC/CCT chaperonin is required for the folding and inhibitory effect of WDTC1 on adipogenesis; Authors - Wen-Shuai Tang
mouse	WB	J Alzheimers Dis; Aging as a Precipitating Factor in Chronic Restraint Stress-Induced Tau Aggregation Pathology, and the Protective Effects of Rosmarinic Acid; Authors - Ye Shan
mouse	IP	Virology; Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.; Authors - Sandhya Gopal
mouse	WB	J Agric Food Chem; A Proteomic Study Reveals a Co-occurrence of Gallic Acid-induced Apoptosis and Glycolysis in B16F10 Melanoma Cells.; Authors - Cheng Liu