Anticorps Monoclonal anti-TCP1

TCP1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C2F6

N° de cat : 68183-1-PBS

Synonymes

D6S230E, Chaperonin containing T-complex polypeptide 1 subunit 1, CCT-alpha, CCTa, CCT1

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Galerie de données de validation

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  WB analysis using 68183-1-Ig (same clone as 68183-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68183-1-Ig (TCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TCP1 antibody (68183-1-Ig, Clone: 2C2F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68183-1-Ig (same clone as 68183-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TCP1 (68183-1-Ig, Clone:2C2F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68183-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68183-1-PBS cible TCP1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TCP1 Protéine recombinante Ag0389
• Nom complet: t-complex 1
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC000665
• Symbole du gène: TCP1
• Identification du gène (NCBI): 6950
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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