Anticorps Recombinant de lapin anti-TCP11L2

TCP11L2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240096F6

N° de cat : 83227-1-RR

Synonymes

T-complex protein 11-like protein 2, t complex 11 (mouse) like 2, 240096F6

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Galerie de données de validation

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  WB analysis using 83227-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 83227-1-RR

  MDCK cells were subjected to SDS PAGE followed by western blot with 83227-1-RR (TCP11L2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 83227-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TCP11L2 antibody (83227-1-RR, Clone: 240096F6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  FC experiment of A431 using 83227-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of SH-SY5Y using 83227-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug TCP11L2 Recombinant antibody (83227-1-RR, Clone:240096F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83227-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83327-1-RR against Human TCP11L2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules MDCK, cellules U251
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules A431, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83227-1-RR cible TCP11L2 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons canin, Humain

• Réactivité: canin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TCP11L2 Protéine recombinante Ag11185
• Nom complet: t-complex 11 (mouse)-like 2
• Masse moléculaire calculée: 519 aa, 58 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC033617
• Symbole du gène: TCP11L2
• Identification du gène (NCBI): 255394
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCP11L2 is a protein containing a serine-rich region in its N-terminal region. It is a member of the TCP11 family, which includes SOK1, TCP11L1, and TCP11L2. TCP11L2 has a role in the migration and differentiation of skeletal muscle-derived satellite cells. It has been shown that Tcp11l2 expression gradually increased during the differentiation of bovine muscle-derived satellite cells. (PMID: 32017087)

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