Anticorps Recombinant de lapin anti-TCTN2

TCTN2 Recombinant Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

6N22

N° de cat : 82120-1-RR

Synonymes

Tectonic-2, tectonic family member 2, Tectonic 2, TECT2, C12orf38

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Galerie de données de validation

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  IHC staining of human colon cancer using 82120-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82120-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82120-1-RR (TCTN2 antibody) at dilution of 1:1050 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82120-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82120-1-RR against Human TCTN2 were performed. The affinity constant is 66 nM.

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:525-1:2100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82120-1-RR cible TCTN2 dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TCTN2 Protéine recombinante Ag10725
• Nom complet: tectonic family member 2
• Masse moléculaire calculée: 697 aa, 77 kDa
• Numéro d’acquisition GenBank: BC009112
• Symbole du gène: TCTN2
• Identification du gène (NCBI): 79867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TCTN2 (tectonic-2 or TECT2) is a type I membrane protein that belongs to the tectonic family, which consists of TCTN1, TCTN2 and TCTN3. Studies in mice suggest that tectonic may be involved in Hedgehog (Hh) signaling, and essential for ciliogenesis. Tectonic is component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. It exists two spilcing isoforms. The first aberrant transcript introduces 104 base pair from intron 13 and would delete 196 original amino acid, introduce two novel amino acids, and prematurely truncate the 697aa protein at residue number 504. The second transcript lacks exon 14 and would delete 195 original amino acids, introduce four novel amino acids, and prematurely truncate the 697 aa protein at residue number 507. The molecular weight of two isoforms are about 50kDa. We detected the 50kDa and 90kDa protein by immunoprecipitaion, but 50kDa protein may be the splicing isoform or non-specific band.

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