Anticorps Polyclonal de lapin anti-TDG

• Phare
• Validé par KD/KO

TDG Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 13370-1-AP

Synonymes

EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase, Thymine-DNA glycosylase

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Galerie de données de validation

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  WB analysis using 13370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13370-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13370-1-AP

  WB result of TDG antibody (13370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDG transfected HEK-293 cells.

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  WB analysis of mouse thymus using 13370-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13370-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13370-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13370-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 13370-1-AP

  IP result of anti-TDG (IP:13370-1-AP, 3ug; Detection:13370-1-AP 1:400) with U-937 cells lysate 3000ug.

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  IF Staining of HeLa using 13370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13370-1-AP (TDG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules MCF-7, cellules Raji, tissu cérébral humain, tissu de côlon de souris, tissu de thymus de souris
• Résultats positifs en IP: cellules U-937
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

13370-1-AP cible TDG dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TDG Protéine recombinante Ag4190
• Nom complet: thymine-DNA glycosylase
• Masse moléculaire calculée: 410 aa, 46 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC037557
• Symbole du gène: TDG
• Identification du gène (NCBI): 6996
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.

Publications

Species	Application	Title
human	WB,IP	J Cell Biol; Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins.; Authors - Umut Sahin
human	WB	Proc Natl Acad Sci U S A; Identification of RING finger protein 4 (RNF4) as a modulator of DNA demethylation through a functional genomics screen.; Authors - Hu Xiaoyi V XV ; KD Validated
human	WB	Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human	IHC,ChIP	J Biol Chem; Thymine DNA Glycosylase is a Positive Regulator of Wnt Signaling in Colorectal Cancer.; Authors - Xuehe Xu
human	WB	J Biol Chem; CRL4Cdt2 E3 Ubiquitin Ligase and PCNA Cooperate to Degrade Thymine DNA Glycosylase in S-phase.; Authors - Etsuko Shibata
human	WB	Cell Death Dis; The HOTAIRM1/miR-107/TDG axis regulates papillary thyroid cancer cell proliferation and invasion.; Authors - Dan Li

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