Anticorps Monoclonal anti-TDP-43

TDP-43 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

1G4F5

N° de cat : 66734-1-Ig

Synonymes

TARDBP, TDP 43, TDP43, 1G4F5, ALS10

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Galerie de données de validation

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  WB analysis using 66734-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66734-1-Ig (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66734-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain, tissu hépatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

66734-1-Ig cible TDP-43 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001487
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

Publications

Species	Application	Title
human		Acta Neuropathol; Neurons selectively targeted in frontotemporal dementia reveal early stage TDP-43 pathobiology.; Authors - Alissa L Nana
mouse	WB	Int J Biol Sci; Spreading of pathological TDP-43 along corticospinal tract axons induces ALS-like phenotypes in Atg5+/- mice.; Authors - Rui Zhang
mouse	IHC	Exp Neurol; Muscle-dominant wild-type TDP-43 expression induces myopathological changes featuring tubular aggregates and TDP-43-positive inclusions.; Authors - Nozomu Tawara
mouse		Sci Rep; A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin.; Authors - Phaneendra Chennampally
human		Nat Commun; TDP-43 proteinopathy in ALS is triggered by loss of ASRGL1 and associated with HML-2 expression; Authors - Marta Garcia-Montojo
human	WB	J Nat Prod; Phenolics and Phenolic Glycosides from Wrightia pubescens and Their Hepatoprotective Activities; Authors - Xingxiang Chen

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