Anticorps Monoclonal anti-TDP-43

TDP-43 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G4F5

N° de cat : 66734-1-PBS

Synonymes

TARDBP, TDP 43, TDP43, 1G4F5, ALS10

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Galerie de données de validation

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  WB analysis using 66734-1-Ig (same clone as 66734-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66734-1-Ig (TDP-43 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66734-1-Ig (same clone as 66734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66734-1-Ig (TDP-43 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66734-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66734-1-PBS cible TDP-43 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001487
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 66734-1-Ig is a mouse monoclonal antibody recognizing the intact protein of TDP-43.

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