Anticorps Monoclonal anti-TDP-43 (C-terminal)

• Phare
• Validé par KD/KO

TDP-43 (C-terminal) Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1G5F6

N° de cat : 67345-1-PBS

Synonymes

TARDBP, TDP 43, TDP43, TDP-43, 1G5F6

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Galerie de données de validation

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  WB analysis of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

  WB result of TDP-43 (C-terminal) antibody (67345-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 (C-terminal) transfected HeLa cells. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  WB analysis using 67345-1-Ig (same clone as 67345-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67345-1-Ig (same clone as 67345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 67345-1-Ig (same clone as 67345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 67345-1-Ig (same clone as 67345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67345-1-Ig (same clone as 67345-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TDP-43 (C-terminal) antibody (67345-1-Ig, Clone: 1G5F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67345-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67345-1-PBS cible TDP-43 (C-terminal) dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: NM_007375
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.

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