Anticorps Polyclonal de lapin anti-TDP1

• Phare
• Validé par KD/KO

TDP1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10641-1-PBS

Synonymes

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, Tyr DNA phosphodiesterase 1, EC:3.1.4.-

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 10641-1-AP (same clone as 10641-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10641-1-AP (same clone as 10641-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10641-1-AP (TDP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10641-1-AP (same clone as 10641-1-PBS)

  IP result of anti-TDP1 (IP:10641-1-AP, 4ug; Detection:10641-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 10641-1-AP (same clone as 10641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 10641-1-AP (same clone as 10641-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10641-1-AP (TDP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10641-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

10641-1-PBS cible TDP1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TDP1 Protéine recombinante Ag0964
• Nom complet: tyrosyl-DNA phosphodiesterase 1
• Masse moléculaire calculée: 34 kDa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC006083
• Symbole du gène: TDP1
• Identification du gène (NCBI): 55775
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tyrosyl DNA phosphodiesterase 1 (TDP1) is an enzyme capable of hydrolyzing phosphodiester bonds between tyrosine and the 3-phosphate of DNA, which are typically generated in a transient manner by DNA topoisomerase I (topo I) and it appears to be more mobile and diffusible than topo I, and it has a different distribution in the nucleus (PMID:15494395). TDP1 is involed in protecting cells against oxidative DNA damage, and with the impaired ability of TDP1-deficient cells to remove 3-phosphoglycolate (PMID:21041670). Defects in TDP1 are the cause of spinocerebellar ataxia autosomal recessive with axonal neuropathy (SCAN1)(PMID:17948061).

{{ptg:RelatedPrimaryAntibodies}}