Anticorps Polyclonal de lapin anti-TEAD3

TEAD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13120-1-PBS

Synonymes

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

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Galerie de données de validation

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  WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

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  WB analysis using 13120-1-AP (same clone as 13120-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13120-1-AP (TEAD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse intestine using 13120-1-AP (same clone as 13120-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 13120-1-AP (TEAD3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 13120-1-AP (same clone as 13120-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13120-1-AP (TEAD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13120-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13120-1-PBS cible TEAD3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TEAD3 Protéine recombinante Ag3875
• Nom complet: TEA domain family member 3
• Masse moléculaire calculée: 435 aa, 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC027877
• Symbole du gène: TEAD3
• Identification du gène (NCBI): 7005
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TEAD3, others named ranscriptional enhancer factor 5(TEF5), a member of family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involve in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in placenta and choriocarcinoma cells(JEG-3), but low in Hela, HepG2 and MCF-7. This is a rabbit polyclonal anti-TEAD3 antibody raised against part of C-terminal chain of human TEAD3.

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