Anticorps Monoclonal anti-TEM1

TEM1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9B4

N° de cat : 60170-1-PBS

Synonymes

CD248, 1F9B4, CD164L1, CD248 molecule, endosialin, Endosialin

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Galerie de données de validation

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  WB analysis using 60170-1-Ig (same clone as 60170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody) at dilution of 1:23000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  WB analysis of HUVEC using 60170-1-Ig (same clone as 60170-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 60170-1-Ig (same clone as 60170-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 60170-1-Ig (TEM1 antibody at dilution of 1:1800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60170-1-Ig (same clone as 60170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60170-1-Ig (TEM1 antibody at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60170-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51052-1

  Cytometric bead array standard curve of MP51052-1, CD248 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60170-1-PBS. Detection antibody: 60170-3-PBS. Standard:Ag13334. Range: 6.25-200 ng/mL.

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Informations sur le produit

60170-1-PBS cible TEM1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TEM1 Protéine recombinante Ag13334
• Nom complet: CD248 molecule, endosialin
• Masse moléculaire calculée: 757 aa, 81 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC051340
• Symbole du gène: TEM1
• Identification du gène (NCBI): 57124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.

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