Anticorps Polyclonal de lapin anti-TERF2IP

TERF2IP Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14595-1-AP

Synonymes

DRIP5, hRap1, RAP1 homolog, TERF2IP

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Galerie de données de validation

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  WB analysis of HEK-293 using 14595-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14595-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14595-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14595-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14595-1-AP

  IP Result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug.

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14595-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 14595-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TERF2IP antibody (14595-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules HT-1080, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

14595-1-AP cible TERF2IP dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TERF2IP Protéine recombinante Ag6130
• Nom complet: telomeric repeat binding factor 2, interacting protein
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC005841
• Symbole du gène: TERF2IP
• Identification du gène (NCBI): 54386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; Preferential extension of short telomeres induced by low extracellular pH.; Authors - Yuanlong Ge
human	WB	Exp Cell Res; C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.; Authors - Radhika Koranne