Anticorps Polyclonal de lapin anti-TEX14

TEX14 Polyclonal Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18351-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18351-1-AP (TEX14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18351-1-AP (same clone as 18351-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18351-1-AP (TEX14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18351-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18351-1-PBS cible TEX14 dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TEX14 Protéine recombinante Ag13177
• Nom complet: testis expressed 14
• Masse moléculaire calculée: 957aa,107 kDa; 1497aa,168 kDa
• Poids moléculaire observé: 180-200 kDa, 106 kDa
• Numéro d’acquisition GenBank: BC040526
• Symbole du gène: TEX14
• Identification du gène (NCBI): 56155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.

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