Anticorps Monoclonal anti-TEX14

TEX14 Monoclonal Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, rat, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11D6

N° de cat : 67982-1-PBS

Synonymes

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14

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Galerie de données de validation

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  WB analysis of rat testis using 67982-1-Ig (same clone as 67982-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit testis using 67982-1-Ig (same clone as 67982-1-PBS)

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 67982-1-Ig (same clone as 67982-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67982-1-Ig (TEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67982-1-Ig (same clone as 67982-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using TEX14 antibody (67982-1-Ig, Clone: 1A11D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67982-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67982-1-PBS cible TEX14 dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, rat, souris

• Réactivité: Humain, Lapin, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TEX14 Protéine recombinante Ag13143
• Nom complet: testis expressed 14
• Masse moléculaire calculée: 957aa,107 kDa; 1497aa,168 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC040526
• Symbole du gène: TEX14
• Identification du gène (NCBI): 56155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment.

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