Anticorps Polyclonal de lapin anti-TEX264

• Phare
• Validé par KD/KO

TEX264 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25858-1-PBS

Synonymes

SIG11, TEG 264, Testis-expressed protein 264, TEX 264, UNQ337/PRO536

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Galerie de données de validation

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  WB analysis using 25858-1-AP (same clone as 25858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 25858-1-AP (same clone as 25858-1-PBS)

  WB result of TEX264 antibody (25858-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX264 transfected HEK-293 cells. This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 25858-1-AP (same clone as 25858-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 25858-1-AP (same clone as 25858-1-PBS)

  IP result of anti-TEX264 (IP:25858-1-AP, 4ug; Detection:25858-1-AP 1:3000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 25858-1-AP (same clone as 25858-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 25858-1-AP (same clone as 25858-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using TEX264 antibody (25858-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25858-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25858-1-PBS cible TEX264 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TEX264 Protéine recombinante Ag23027
• Nom complet: testis expressed 264
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC008742
• Symbole du gène: TEX264
• Identification du gène (NCBI): 51368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

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