Anticorps Recombinant de lapin anti-TEX264

TEX264 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242500B3

N° de cat : 84946-5-PBS

Synonymes

242500B3, SIG11, TEG 264, Testis-expressed protein 264, TEX 264

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Galerie de données de validation

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  WB analysis using 84946-5-RR (same clone as 84946-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84946-5-RR (TEX264 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 84946-5-RR (same clone as 84946-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84946-5-RR (TEX264 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

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  IF Staining of Neuro-2a using 84946-5-RR (same clone as 84946-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TEX264 antibody (84946-5-RR, Clone: 242500B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84946-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84946-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84946-5-RR against Human TEX264 were performed. The affinity constant is 0.254 nM.

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Informations sur le produit

84946-5-PBS cible TEX264 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TEX264 Protéine recombinante Ag23027
• Nom complet: testis expressed 264
• Masse moléculaire calculée: 313 aa, 34 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC008742
• Symbole du gène: TEX264
• Identification du gène (NCBI): 51368
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

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