Anticorps Monoclonal anti-TFAM

• Phare
• Validé par KD/KO

TFAM Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8H3

N° de cat : 68557-5-PBS

Synonymes

MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2

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Galerie de données de validation

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  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68557-5-Ig (same clone as 68557-5-PBS)

  WB result of TFAM antibody (68557-5-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAM transfected HEK-293 cells. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  WB analysis using 68557-5-Ig (same clone as 68557-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68557-5-Ig (TFAM antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68557-5-Ig (TFAM antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68557-5-Ig (same clone as 68557-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAM antibody (68557-5-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 68557-5-PBS in a different storage buffer formulation.

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Informations sur le produit

68557-5-PBS cible TFAM dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFAM Protéine recombinante Ag18253
• Nom complet: transcription factor A, mitochondrial
• Masse moléculaire calculée: 246 aa, 29 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC126366
• Symbole du gène: TFAM
• Identification du gène (NCBI): 7019
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.

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