Anticorps Polyclonal de lapin anti-TFAP2A/AP-2

• Phare
• Validé par KD/KO

TFAP2A/AP-2 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 13019-3-AP

Synonymes

TFAP2A, TFAP2A,AP-2, AP-2 transcription factor, AP-2, AP2

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Galerie de données de validation

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  WB analysis using 13019-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13019-3-AP (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13019-3-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13019-3-AP (TFAP2A/AP-2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 13019-3-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TFAP2A/AP-2 antibody (13019-3-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, cellules Y79
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

13019-3-AP cible TFAP2A/AP-2 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFAP2A/AP-2 Protéine recombinante Ag4112
• Nom complet: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC017754
• Symbole du gène: TFAP2A/AP-2
• Identification du gène (NCBI): 7020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression (PMID: 21966377). TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation (PMID: 11694877).

Publications

Species	Application	Title
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
mouse	WB,IHC	J Ethnopharmacol; Erzhi Pill promotes norepinephrine synthesis in locus coeruleus through ERβ-TFAP2A-TH plays a neuroprotective role; Authors - Ao Xue
		Oncol Rep; Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.; Authors - Ming-Xu Qiao
human, mouse	WB, ChIP	Acta Pharmacol Sin; Hepatitis B virus X protein promotes human hepatoma cell growth via upregulation of transcription factor AP2α and sphingosine kinase 1.; Authors - Zhan-Ping Lu ; KD Validated
human	WB	Cancer Cell Int; HNRNPC promotes progression of non-small cell lung cancer by maintaining TFAP2A mRNA stability; Authors - Minghua Liao
	ChIP	Cell Death Dis; TFAP2A potentiates lung adenocarcinoma metastasis by a novel miR-16 family/TFAP2A/PSG9/TGF-β signaling pathway.; Authors - Yanlu Xiong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good IF antibody. no unspecific staining

• Applications: Immunofluorescence
• Cell Tissue Type: Retinal organoids

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