Anticorps Monoclonal anti-TFAP2A/AP-2

TFAP2A/AP-2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1B12F11

N° de cat : 67076-1-Ig

Synonymes

TFAP2A, TFAP2A,AP-2, 1B12F11, Activating enhancer-binding protein 2-alpha, AP 2

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Galerie de données de validation

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  WB analysis of L02 using 67076-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67076-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67076-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67076-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67076-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67076-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 67076-1-Ig

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TFAP2A/AP-2 (67076-1-Ig, Clone:1B12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below

Informations sur le produit

67076-1-Ig cible TFAP2A/AP-2 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFAP2A/AP-2 Protéine recombinante Ag4112
• Nom complet: transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
• Masse moléculaire calculée: 431 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC017754
• Symbole du gène: TFAP2A/AP-2
• Identification du gène (NCBI): 7020
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]

Publications

Species	Application	Title
human	IHC	Cancer Cell Int; HNRNPC promotes progression of non-small cell lung cancer by maintaining TFAP2A mRNA stability; Authors - Minghua Liao
human	IHC	Front Genet; Identifification and validation of ferroptosis signatures and immune infifiltration characteristics associated with intervertebral disc degeneration; Authors - Feng Zhang
human	IHC	Oncol Rep; TFAP2A promotes cervical cancer via a positive feedback pathway with PD‑L1; Authors - Junyuan Yang

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