Anticorps Recombinant de lapin anti-TFAP2C/AP2 gamma

TFAP2C/AP2 gamma Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250904E9

N° de cat : 86365-1-RR

Synonymes

TFAP2C, Activating enhancer-binding protein 2 gamma, ap 2gamma, AP2 GAMMA, AP2-gamma

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Galerie de données de validation

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  WB analysis using 86365-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86365-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86365-1-RR (TFAP2C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86365-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TFAP2C antibody (86365-1-RR, Clone: 250904E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A431, cellules PC-3
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86365-1-RR cible TFAP2C/AP2 gamma dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TFAP2C/AP2 gamma Protéine recombinante Ag5648
• Nom complet: transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC035664
• Symbole du gène: TFAP2C
• Identification du gène (NCBI): 7022
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFAP2C (Transcription Factor AP-2 Gamma) is a member of the AP-2 transcription factor family, which plays a crucial role in early embryonic development, specifically in regulating the development of trophoblast cells and maintaining pluripotency. TFAP2C is known to regulate transcription by opening enhancers and is associated with a set of enhancers present in human embryos. It also functions as a regulator of both inner cell mass and trophectoderm differentiation, and controls the expression of cell-polarity genes. This protein binds to specific DNA sequences to activate or repress target genes, making it a key player in cellular processes and a potential therapeutic target in oncology research.

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