Anticorps Polyclonal de lapin anti-TFDP2

TFDP2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 11500-1-AP

Synonymes

Transcription factor Dp-2, DP2, Dp 2

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Galerie de données de validation

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  WB analysis of mouse heart using 11500-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11500-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:400) with SH-SY5Y cells lysate 1600ug.

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  IP experiment of SH-SY5Y using 11500-1-AP

  IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:600) with SH-SY5Y cells lysate 1360 ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral humain
• Résultats positifs en IP: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

11500-1-AP cible TFDP2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFDP2 Protéine recombinante Ag2054
• Nom complet: transcription factor Dp-2 (E2F dimerization partner 2)
• Masse moléculaire calculée: 446 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC021113
• Symbole du gène: TFDP2
• Identification du gène (NCBI): 7029
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].

Publications

Species	Application	Title
mouse	WB	Mol Syst Biol; The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.; Authors - Caron Etienne E
human	IHC	Front Mol Biosci; Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data; Authors - Ya-Jun Deng
	WB	Neuropharmacology; The Novel miR_146-Tfdp2 Axis Antagonizes METH-Induced Neuron Apoptosis and Cell Cycle Abnormalities in Tree Shrew; Authors - Shuwei Zhang

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