Anticorps Recombinant de lapin anti-TFEB

TFEB Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242093D11

N° de cat : 84675-5-RR

Synonymes

242093D11, Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35

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Galerie de données de validation

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  WB analysis using 84675-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84675-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (84675-5-RR, Clone: 242093D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84675-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84675-5-RR cible TFEB dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TFEB Protéine recombinante Ag4194
• Nom complet: transcription factor EB
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC032448
• Symbole du gène: TFEB
• Identification du gène (NCBI): 7942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

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