Anticorps Polyclonal de lapin anti-TFEB

• Phare
• Validé par KD/KO

TFEB Polyclonal Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(1)

N° de cat : CL594-13372

Synonymes

AlphaTFEB, bHLHe35, TCFEB, TFEB, transcription factor EB

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Galerie de données de validation

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  IF Staining of HepG2 using CL594-13372

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 TFEB antibody (CL594-13372) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-13372

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 TFEB antibody (CL594-13372) at dilution of 1:600.

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL594-13372 cible TFEB dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFEB Protéine recombinante Ag4194
• Nom complet: transcription factor EB
• Masse moléculaire calculée: 476 aa, 53 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC032448
• Symbole du gène: TFEB
• Identification du gène (NCBI): 7942
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calcualted molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

Publications

Species	Application	Title
	IF	Genet Med; De novo missense variants in RRAGC lead to a fatal mTORopathy of early childhood; Authors - Margot R F Reijnders

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