Anticorps Polyclonal de lapin anti-Trefoil factor 1

• Phare
• Validé par KD/KO

Trefoil factor 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 13734-1-AP

Synonymes

D21S21, HP1.A, HPS2, pNR 2, Polypeptide P1.A, Protein pS2, pS2, TFF1, trefoil factor 1

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Galerie de données de validation

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  WB analysis of human stomach using 13734-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 13734-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13734-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 13734-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 13734-1-AP (Trefoil factor 1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13734-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 13734-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Trefoil factor 1 antibody (13734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu d'estomac humain, tissu d'estomac de souris, tissu rénal humain
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de l'estomac humain, tissu de tumeur ovarienne humain, tissu d'estomac de rat, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

13734-1-AP cible Trefoil factor 1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Trefoil factor 1 Protéine recombinante Ag4541
• Nom complet: trefoil factor 1
• Masse moléculaire calculée: 84 aa, 9 kDa
• Poids moléculaire observé: 9-12 kDa
• Numéro d’acquisition GenBank: BC032811
• Symbole du gène: TFF1
• Identification du gène (NCBI): 7031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFF1 (formerly known as pS2) is a small-secreted protein that belongs to the family of trefoil peptides, and characterized by the presence of one to six cysteine-rich P domains. TFF1 expression is frequently lost in gastric carcinoma, probably through mechanism of DNA methylation, and it is therefore considered as a tumor suppressor gene. The function of TFF1 was first controversially considered as an oncogene in breast cancer.

Publications

Species	Application	Title
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
human	IHC	JCI Insight; Resolving the difference between left-sided and right-sided colorectal cancer by single-cell sequencing.; Authors - Wei Guo
human	IHC, WB	Bone; Trefoil factor-1 upregulation in estrogen-receptor positive breast cancer correlates with an increased risk of bone metastasis.; Authors - Chiara Spadazzi ; KO Validated
human	WB, IHC	Biomed Pharmacother; Trefoil factor 1 (TFF1) is a potential prognostic biomarker with functional significance in breast cancers.; Authors - Jie Yi ; KD Validated
human	WB	Aesthet Surg J; Cell-Free Fat Extract Prevents Vaginal Atrophy in Ovariectomized Model Via Promoting Proliferation of Vaginal Keratinocyte and Neovascularization.; Authors - Bijun Kang
human	IHC	BMC Med Genomics; Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.; Authors - Kim Hark Kyun HK