Anticorps Polyclonal de lapin anti-TFF2

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13681-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  WB analysis using 13681-1-AP (same clone as 13681-1-PBS)

  Human urine tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  human stomach sample were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13681-1-AP (TFF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 13681-1-AP (same clone as 13681-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13681-1-AP (TFF2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13681-1-PBS cible TFF2 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFF2 Protéine recombinante Ag4537
• Nom complet: trefoil factor 2
• Masse moléculaire calculée: 129 aa, 14 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC032820
• Symbole du gène: TFF2
• Identification du gène (NCBI): 7032
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

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