Anticorps Recombinant de lapin anti-TFF2

TFF2 Recombinant Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

240661G12

N° de cat : 83717-4-RR

Synonymes

SML1, 240661G12, trefoil factor 2, Spasmolytic polypeptide, Spasmolysin

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Galerie de données de validation

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  WB analysis of human urine using 83717-4-RR

  Human urine sample was subjected to SDS PAGE followed by western blot with 83717-4-RR (TFF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 83717-4-RR

  IP result of anti-TFF2 (IP:83717-4-RR, 4ug; Detection:83717-4-RR 1:1000) with mouse stomach tissue lysate 1360 ug.

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  FC experiment of PC-3 using 83717-4-RR

  1x10^6 PC-3 cells were intracellularly stained with 0.25 ug TFF2 Recombinant antibody (83717-4-RR, Clone:240661G12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83717-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83717-4-RR against Human TFF2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: échantillon d'urine humaine,
• Résultats positifs en IP: tissu d'estomac de souris,
• Résultats positifs en FC (Intra): cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83717-4-RR cible TFF2 dans les applications de WB, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TFF2 Protéine recombinante Ag4537
• Nom complet: trefoil factor 2
• Masse moléculaire calculée: 129 aa, 14 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC032820
• Symbole du gène: TFF2
• Identification du gène (NCBI): 7032
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

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