Anticorps Polyclonal de lapin anti-TFG

• Phare
• Validé par KD/KO

TFG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 11571-1-AP

Synonymes

TRKT3, TRK-fused gene protein, TRK fused gene protein, TRK fused gene, TF6

---

Galerie de données de validation

• 

  WB analysis using 11571-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11571-1-AP (TFG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11571-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11571-1-AP (TFG antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of A549 using 11571-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11571-1-AP (TFG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules PC-3
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

11571-1-AP cible TFG dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFG Protéine recombinante Ag2151
• Nom complet: TRK-fused gene
• Masse moléculaire calculée: 400 aa, 43 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC023599
• Symbole du gène: TFG
• Identification du gène (NCBI): 10342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.

Publications

Species	Application	Title
human	WB,IF	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
mouse	WB	Neurobiol Dis; Evidence of TRK-Fused Gene (TFG1) function in the ubiquitin-proteasome system.; Authors - Takuya Yagi ; KD Validated
human	IF	J Med Genet; Novel TFG mutation causes autosomal-dominant spastic paraplegia and defects in autophagy; Authors - Ling Xu
human	IF	Muscle Nerve; Muscle pathology of hereditary motor and sensory neuropathy with proximal dominant involvement with TFG mutation.; Authors - Satoshi Yamashita
	WB	Commun Biol; A pore-forming protein drives macropinocytosis to facilitate toad water maintaining.; Authors - Zhong Zhao

{{ptg:RelatedPrimaryAntibodies}}