Anticorps Monoclonal anti-TFG

TFG Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B5B9

N° de cat : 66916-1-Ig

Synonymes

FLJ36137, Protein TFG, TF6, TFG, TRK fused gene, TRK fused gene protein, TRKT3

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Galerie de données de validation

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  WB analysis using 66916-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66916-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 66916-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66916-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66916-1-Ig (TFG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66916-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66916-1-Ig (TFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellules A549, cellules HEK-293, cellules LNCaP, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66916-1-Ig cible TFG dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFG Protéine recombinante Ag27697
• Nom complet: TRK-fused gene
• Masse moléculaire calculée: 400 aa, 43 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC023599
• Symbole du gène: TFG
• Identification du gène (NCBI): 10342
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein TFG (TRK-fused gene protein) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. Mutations in TFG may have important clinical relevance for current therapeutic strategies to treat metastatic melanoma. Defects in TFG are a cause of thyroid papillary carcinoma (TPC), a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, and atrophy followed by distal sensory involvement. Recent genetic investigation indicates that formation of TFG-containing cytoplasmic inclusions and concomitant mislocalization of TAR DNA-binding protein 43 kDa (TDP-43) underlie motor neuron degeneration in HMSN-P. Pathological overlap of proteinopathies involving TFG and TDP-43 highlights a new pathway leading to motor neuron degeneration.

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