Anticorps Monoclonal anti-TFIP11

TFIP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A1C8

N° de cat : 66114-1-PBS

Synonymes

1A1C8, bK445C9.6, hNtr1, HSPC006, NTR1

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Galerie de données de validation

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  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66114-1-Ig (same clone as 66114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66114-1-Ig (same clone as 66114-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66114-1-Ig (same clone as 66114-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66114-1-Ig (same clone as 66114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66114-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66114-1-PBS cible TFIP11 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFIP11 Protéine recombinante Ag5821
• Nom complet: tuftelin interacting protein 11
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC033080
• Symbole du gène: TFIP11
• Identification du gène (NCBI): 24144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

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