Anticorps Monoclonal anti-TFPI

TFPI Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1B12F11

N° de cat : 66842-1-Ig

Synonymes

EPI, Extrinsic pathway inhibitor, LACI, TFI, TFPI, TFPI1

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Galerie de données de validation

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  WB analysis of human placenta using 66842-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66842-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

66842-1-Ig cible TFPI dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFPI Protéine recombinante Ag14980
• Nom complet: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Masse moléculaire calculée: 251aa,29 kDa; 304aa,35 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC015514
• Symbole du gène: TFPI
• Identification du gène (NCBI): 7035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer.; Authors - Ting Li