Anticorps Monoclonal anti-TFPI

TFPI Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B12F11

N° de cat : 66842-1-PBS

Synonymes

1B12F11, EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor

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Galerie de données de validation

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  WB analysis of human placenta using 66842-1-Ig (same clone as 66842-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66842-1-Ig (same clone as 66842-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66842-1-Ig (TFPI antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66842-1-Ig (same clone as 66842-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TFPI antibody (66842-1-Ig, Clone: 1B12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66842-1-Ig (same clone as 66842-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TFPI antibody (66842-1-Ig, Clone: 1B12F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66842-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66842-1-PBS cible TFPI dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TFPI Protéine recombinante Ag14980
• Nom complet: tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
• Masse moléculaire calculée: 251aa,29 kDa; 304aa,35 kDa
• Poids moléculaire observé: 35-38 kDa
• Numéro d’acquisition GenBank: BC015514
• Symbole du gène: TFPI
• Identification du gène (NCBI): 7035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases.

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