Anticorps Polyclonal de lapin anti-TFPT

TFPT Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 10097-2-AP

Synonymes

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

---

Galerie de données de validation

• 

  WB analysis of K-562 using 10097-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10097-2-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10097-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10097-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 10097-2-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 10097-2-AP (TFPT Antibody) at dilution of 1:50.

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HL-60, cellules PC-3, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

10097-2-AP cible TFPT dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TFPT Protéine recombinante Ag0131
• Nom complet: TCF3 (E2A) fusion partner (in childhood Leukemia)
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC001728
• Symbole du gène: TFPT
• Identification du gène (NCBI): 29844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

Publications

Species	Application	Title
mouse	WB	EMBO J; LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling.; Authors - Yanying Wang
mouse	WB	EMBO J; Linc-MYH configures INO80 to regulate muscle stem cell numbers and skeletal muscle hypertrophy.; Authors - Christian Schutt

{{ptg:RelatedPrimaryAntibodies}}