Anticorps Recombinant de lapin anti-TGF Beta 1

TGF Beta 1 Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

PE Fluorescent Dye

CloneNo.

230544B7

N° de cat : PE-81746-2

Synonymes

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

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Galerie de données de validation

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  IF Staining of HEK-293 using PE-81746-2

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PE TGF Beta 1 antibody (PE-81746-2, Clone: 230544B7 ) at dilution of 1:200.

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  FC experiment of human PBMCs using PE-81746-2

  1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.2 ug PE-conjugated TGF Beta 1 Recombinant antibody (PE-81746-2, Clone: 230544B7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of HEK-293 using PE-81746-2

  1x10^6 HEK-293 cells were intracellularly stained with 0.1 ug PE TGF Beta 1 Recombinant Antibody (PE-81746-2, Clone: 230544B7) (red) or PE Rabbit IgG Isotype Control RecAb (PE-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules HEK-293,
• Résultats positifs en FC (Intra): PHA treated human PBMCs, cellules HEK-293

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-81746-2 cible TGF Beta 1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGF Beta 1 Protéine recombinante Ag13591
• Nom complet: transforming growth factor, beta 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000125
• Symbole du gène: TGFB1
• Identification du gène (NCBI): 7040
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul contiennent 0,1% de BSA.

Informations générales

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.

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