Anticorps Polyclonal de lapin anti-TGF beta 1

TGF beta 1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(16)

N° de cat : 26155-1-AP

Synonymes

Tgfb1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, Latency-associated peptide, TGF beta1, Tgfb

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Galerie de données de validation

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  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26155-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26155-1-AP (TGF beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26155-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 26155-1-AP

  NIH3T3 cells were subjected to SDS PAGE followed by western blot with 26155-1-AP (Tgfb1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 26155-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TGF beta 1 antibody (26155-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of NIH/3T3 using 26155-1-AP

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug TGF beta 1 Polyclonal antibody (26155-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules L-929, C6, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IF/ICC: cellules NIH/3T3,
• Résultats positifs en FC (Intra): cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

26155-1-AP cible TGF beta 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGF beta 1 Protéine recombinante Ag24078
• Nom complet: transforming growth factor, beta 1
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: NM-011577
• Symbole du gène: TGF beta 1
• Identification du gène (NCBI): 21803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGF beta 1 is a potent cytokine capable of regulating multiple cellular processes including cell proliferation, differentiation, wound healing, and immune response. TGF beta 1 is identified in various immune cells and its activity varies in different cell types or cells at different developmental stages1. TGF beta 1 functions by binding to the TGFβ1 receptors, which subsequently activate the canonical SMAD pathway or non-canonical signaling cascades, such as the mitogen-activated protein kinase (MAPK) pathway, phosphatidylinositol-3 kinase and AKT (PI3K-AKT), and Rho family of GTPases (Rho GTPase). What is special about TGF beta 1 is its context-dependent nature that is particularly true for tumors. Full-length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).

Publications

Species	Application	Title
human	WB	Ecotoxicol Environ Saf; Ambient fine particulate matter induces cardiac fibrosis through triggering ferroptosis by heme degradation induced-iron overload; Authors - Jinjin Jiang
human	WB	Commun Biol; SEC24D depletion induces osteogenic differentiation deficiency by inactivating the ATF6/TGF-β/Runx2 regulatory loop; Authors - Jing Zhang
human	WB	Korean Circ J; LncRNA uc003pxg.1 Interacts With miR-339-5p Promote Vascular Endothelial Cell Proliferation, Migration and Angiogenesis; Authors - Ping Li
pig	WB	Food Funct; Sesamin improved growth and overall health in young animals by enhancing gut-liver axis function; Authors - Chen Chen
rat	WB	Front Pharmacol; Investigating the regulation of the miR-199a-3p/TGF-β/Smad signaling pathway by BSHXF drug-containing serum combined with ADSCs for delaying intervertebral disc degeneration; Authors - Enxu Liu
human	WB,IF	Cell Signal; The role of α7nAchR and PD-L1 in neuroimmune regulation of keloid treatment; Authors - Zucheng Luo

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