Anticorps Recombinant de lapin anti-TGF Beta 1

• Phare
• Validé par KD/KO

TGF Beta 1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230544B7

N° de cat : 81746-2-PBS

Synonymes

TGFB1, TGFB1,TGF beta 1,TGFbeta,TGF-beta 1, TGF-beta1, 230544B7, Latency-associated peptide

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Galerie de données de validation

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  WB analysis of HepG2 using 81746-2-RR (same clone as 81746-2-PBS)

  WB result of TGF Beta 1 antibody (81746-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGF Beta 1 transfected HepG2 cells. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81746-2-RR (same clone as 81746-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81746-2-RR (TGFB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 81746-2-RR (same clone as 81746-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 81746-2-RR (TGF Beta 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGFB1 antibody (81746-2-RR, Clone: 230544B7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 81746-2-RR (same clone as 81746-2-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug TGF Beta 1 Recombinant antibody (81746-2-RR, Clone:230544B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81746-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81746-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81746-2-RR against Human TGFB1 were performed. The affinity constant is 9.65 nM.

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Informations sur le produit

81746-2-PBS cible TGF Beta 1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGF Beta 1 Protéine recombinante Ag13591
• Nom complet: transforming growth factor, beta 1
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC000125
• Symbole du gène: TGFB1
• Identification du gène (NCBI): 7040
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.

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