Anticorps Recombinant de lapin anti-HIC5

HIC5 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250139E8

N° de cat : 85859-5-RR

Synonymes

Androgen receptor-associated protein of 55 kDa, ARA55, Hic-5, Hydrogen peroxide-inducible clone 5 protein, TGFB1I1

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Galerie de données de validation

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  WB analysis of HeLa using 85859-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 85859-5-RR

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 85859-5-RR

  rat colon tissue were subjected to SDS PAGE followed by western blot with 85859-5-RR (HIC5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85859-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85859-5-RR against Human HIC5 were performed. The affinity constant is 6.15 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules SKOV-3, tissu de côlon de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85859-5-RR cible HIC5 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HIC5 Protéine recombinante Ag0868
• Nom complet: transforming growth factor beta 1 induced transcript 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC001830
• Symbole du gène: HIC5
• Identification du gène (NCBI): 7041
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HIC5 (Hydrogen Peroxide-Inducible Clone 5), also known as TGFB1I1, ARA55, or TSC-5. It was first identified in 1994 as a gene induced by transforming growth factor-β (TGF-β) or hydrogen peroxide (PMID: 36222304). HIC5 is a member of the Paxillin protein family and functions as a molecular adapter, delivering signals when the cellular adhesion environment changes. Given its role in cancer and fibrotic diseases, HIC5 is a potential therapeutic target. For example, targeting HIC5 or the GR tau2 region could modulate the set of genes regulated by GR, providing opportunities for intervention (PMID: 24591583).

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