Anticorps Polyclonal de lapin anti-TGF beta 2-Specific

• Phare
• Validé par KD/KO

TGF beta 2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(57)

Avis (2)

N° de cat : 19999-1-AP

Synonymes

TGFB2, TGF-beta 2, Glioblastoma-derived T-cell suppressor factor, G-TSF, Latency-associated peptide

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Galerie de données de validation

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  WB analysis using 19999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19999-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 19999-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19999-1-AP (TGF-beta 2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 19999-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19999-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19999-1-AP (TGF beta 2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 19999-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 19999-1-AP (TGF-beta 2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, cellules MCF-7, cellules MDA-MB-231, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 47 publications below
• IHC: See 14 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Informations sur le produit

19999-1-AP cible TGF beta 2-Specific dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transforming growth factor, beta 2
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: NM_003238
• Symbole du gène: TGFB2
• Identification du gène (NCBI): 7042
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth. This antibody is TGFB2 specific not detecting other TGFBs.

Publications

Species	Application	Title
human	WB	ACS Nano; Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling.; Authors - Na Huang
human	WB,IF	Cancer Res; Osteoblast-secreted factors mediate dormancy of metastatic prostate cancer in the bone via activation of the TGFβRIII-p38MAPK-pS249/T252RB pathway.; Authors - Li-Yuan Yu-Lee ; KD Validated
human	WB	Cell Death Differ; USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.; Authors - Xiaohua Jie
human	WB	Dev Cell; Endothelial-to-Osteoblast Conversion Generates Osteoblastic Metastasis of Prostate Cancer.; Authors - Song-Chang Lin
human	IHC	Cancer Res; SIX1 promotes tumor lymphangiogenesis by coordinating TGFβ signals that increase expression of VEGF-C.; Authors - Dan Liu
human	WB	Cancer Lett; ZEB1 represses biogenesis of circ-DOCK5 to facilitate metastasis in esophageal squamous cell carcinoma via a positive feedback loop with TGF-β.; Authors - Lingjiao Meng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aline Seiko (Verified Customer) (05-12-2025)

It was observed a clear expression of the protein in the expected molecular weight of 48kDa, but also for the spleen it was observed a monomer isoform of 12kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse kidney and spleen lysate

FH

Kazu (Verified Customer) (12-14-2022)

A clear, single band at the expected molecular weight was observed by western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Feline ocular tissue

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