Anticorps Recombinant de lapin anti-TGFBI/BIGH3

TGFBI/BIGH3 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243108F7

N° de cat : 80805-4-RR

Synonymes

TGFBI, TGFBI / BIGH3, Beta ig h3, Beta ig-h3, BIGH3

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Galerie de données de validation

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  WB analysis of HeLa using 80805-4-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 80805-4-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 80805-4-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 80805-4-RR (beta IG-H3/TGFBI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80805-4-RR

  Biolayer interferometry (BLl) kinetic assays of 80805-4-RR against Human TGFBI/BIGH3 were performed. The affinity constant is 1.65 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80805-4-RR cible TGFBI/BIGH3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGFBI/BIGH3 Protéine recombinante Eg1233
• Nom complet: transforming growth factor, beta-induced, 68kDa
• Masse moléculaire calculée: 75kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: NM_000358.3
• Symbole du gène: TGFBI
• Identification du gène (NCBI): 7045
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

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