Anticorps Polyclonal de lapin anti-TGFBR1

TGFBR1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 30117-1-AP

Synonymes

TGFβ RI, AAT5, Activin A receptor type II-like protein kinase of 53kD, Activin receptor like kinase 5, Activin receptor-like kinase 5

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Galerie de données de validation

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  WB analysis using 30117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 30117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30117-1-AP (TGFBR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 30117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 30117-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30117-1-AP (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse ovary using 30117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse ovary tissue using TGFBR1 antibody (30117-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MDA-MB-231, cellules MG U-87, cellules NCI-H1299, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu ovarien de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 3 publications below

Informations sur le produit

30117-1-AP cible TGFBR1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TGFBR1 Protéine recombinante Ag31620
• Nom complet: transforming growth factor, beta receptor 1
• Masse moléculaire calculée: 56KD
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: NM_004612
• Symbole du gène: TGFBR1
• Identification du gène (NCBI): 7046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).

Publications

Species	Application	Title
mouse	IF	Theranostics; Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction; Authors - Meng Gao
mouse	WB	J Nanobiotechnology; Determining M2 macrophages content for the anti-tumor effects of metal-organic framework-encapsulated pazopanib nanoparticles in breast cancer; Authors - Zhijie Xu
mouse	IF	Nat Commun; Targeting the transmembrane cytokine co-receptor neuropilin-1 in distal tubules improves renal injury and fibrosis; Authors - Yinzheng Li
human	WB	Mol Cell Biochem; Lnc PVT1 facilitates TGF-β1-induced human cardiac fibroblast activation in vitro and ISO-induced myocardial fibrosis in vivo through regulating MYC; Authors - Juan Wang
human	WB	Front Pharmacol; Isoliensinine suppressed gastric cancer cell proliferation and migration by targeting TGFBR1 to regulate TGF-β-smad signaling pathways; Authors - Jinda Hu
human	WB	Epigenetics; ANRIL upregulates TGFBR1 to promote idiopathic pulmonary fibrosis in TGF-β1-treated lung fibroblasts via sequestering let-7d-5p; Authors - Weidong Wu

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