Anticorps Recombinant de lapin anti-TGFBR1

TGFBR1 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

241441A11

N° de cat : 84453-1-RR

Synonymes

Activin receptor-like kinase 5, Activin receptor like kinase 5, Activin A receptor type II-like protein kinase of 53kD, AAT5, 241441A11

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Galerie de données de validation

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  WB analysis using 84453-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84453-1-RR (TGFBR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84453-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84453-1-RR (TGFBR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84453-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84453-1-RR against Human TGFBR1 were performed. The affinity constant is 67.5 pM.

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, cellules A549, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84453-1-RR cible TGFBR1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TGFBR1 Protéine recombinante Ag31620
• Nom complet: transforming growth factor, beta receptor 1
• Masse moléculaire calculée: 56KD
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: NM_004612
• Symbole du gène: TGFBR1
• Identification du gène (NCBI): 7046
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TGFBR1 (TGF-beta receptor type-1) encodes a serine/threonine kinase receptor for transforming growth factor-beta. TGFB1, TGFB2 and TGFB3 signals are transduced from the cell surface to the cytoplasm and regulate lots of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Mutations in both TGFBR2 and TGFBR1 were associated with early onset and aggressive thoracic aortic disease with MFS-like skeletal features, but also hypertelorism, craniosynostosis, developmental delay, cleft palate and bifid uvula, congenital heart disease and aneurysms, and dissections throughout the arterial tree with marked arterial tortuosity (PMID: 15731757, PMID: 27879313).

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