Anticorps Monoclonal anti-TGFBR2

• Phare
• Validé par KD/KO

TGFBR2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2D5H7

N° de cat : 66636-1-PBS

Synonymes

2D5H7, EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II

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Galerie de données de validation

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  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis using 66636-1-Ig (same clone as 66636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 66636-1-Ig (same clone as 66636-1-PBS)

  WB result of TGFBR2 antibody (66636-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TGFBR2 transfected HEK-293T cells. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 66636-1-Ig (same clone as 66636-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66636-1-Ig (same clone as 66636-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66636-1-Ig (same clone as 66636-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66636-1-Ig (same clone as 66636-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66636-1-Ig (TGFBR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66636-1-Ig (same clone as 66636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66636-1-Ig (TGFBR2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66636-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66636-1-PBS cible TGFBR2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TGFBR2 Protéine recombinante Ag25773
• Nom complet: transforming growth factor, beta receptor II (70/80kDa)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 65-80 kDa
• Numéro d’acquisition GenBank: BC040499
• Symbole du gène: TGFBR2
• Identification du gène (NCBI): 7048
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). The calculated molecular weight of TGFBR2 is 65 kDa. It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

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